The Effects of High Glucose on Adipogenic and Osteogenic Differentiation of Gestational Tissue-Derived MSCs

Table 1

The sequences of primers for qRT-PCR.

Gene	Forward primer	Reverse primer
ADIPOQ	5′-CCTGGTGAGAAGGGTGAGAA-3′	5′-CAATCCCACACTGAATGCTG-3′
GLUT4	5′-CTTCGAGACAGCAGGGGTAG-3′	5′-ACAGTCATCAGGATGGCACA-3′
LPL	5′-TCAACTGGATGGAGGAGGAG-3′	5′-GGGGCTTCTGCATACTCAAA-3′
PPARγ	5′-GACCACTCCCACTCCTTTGA-3′	5′-AGGCTCCACTTTGATTGCAC-3′
SREBP1C	5′-TTCTCACCTCCCAGCTCTGT-3′	5′-GGAGGCTTCTTTGCTGTGAG-3′
RUNX2	5′-GACAGCCCCAACTTCCTGT-3′	5′-CCGGAGCTCAGCAGAATAAT-3′
OCN	5′-CTCACACTCCTCGCCCTATT-3′	5′-TCAGCCAACTCGTCACAGTC-3′
OSX	5′-TGCTTGAGGAGGAAGTTCAC-3′	5′-CTGCTTTGCCCAGAGTTGTT-3′
GAPDH	5′-CAATGACCCCTTCATTGACC-3′	5′-TTGATTTTGGAGGGATCTCG-3′

ADIPOQ: adiponectin, GLUT4: glucose transporter type 4, LPL: lipoprotein lipase, PPARγ: peroxisome proliferator-activated receptor gamma, SREBP1C: sterol regulatory element-binding protein 1C, RUNX2: runt-related transcription factor 2, OCN: osteocalcin, OSX: osterix, and GAPDH: glyceraldehyde 3-phosphate dehydrogenase.