Comparative Microarray Analysis of Proliferating and Differentiating Murine ENS Progenitor Cells

Figure 1

Time schedule of enterosphere culture. The timeline illustrates the schedule of in vitro culture. Cells were isolated at 0 div (days in vitro) and proliferated for 5 days. Spheres were then picked and again proliferated for 4 days. At 9 div, enterospheres were picked, washed, transferred to differentiation medium, and incubated for 2 days before gene expression analyses were carried out. The micrographs show proliferating enterospheres after 1 and 5 div. Scale bar: 200 μm.