Hepatoma-Derived Growth Factor Secreted from Mesenchymal Stem Cells Reduces Myocardial Ischemia-Reperfusion Injury

Figure 4

HDGF reduced apoptosis and activated PKCε pathway. H9C2 cells treated by recombinant mouse HDGF (100 nmol/L) or vehicle control were subjected to 9 h of hypoxia followed by 4 h of reoxygenation. (a) Cell death was evaluated with flow cytometry analysis. (b) Cleaved caspases-3 as detected by Western blotting. Data represent mean ± SEM of values from three determinations. (c) Phosphorylation of PKCε as detected by Western blotting. Data represent mean ± SEM of values from three determinations. (d) Mitochondria were isolated from H9C2 cells and activity of ALDH2 in mitochondria was measured. Data represent mean ± SEM of values from three mice. (e) 4-HNE protein adducts in H9C2 cells were assessed. Data represent mean ± SEM of values from four determinations. .

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