Skeletal Muscle Cell Induction from Pluripotent Stem Cells

Figure 4

Myogenic cells induced from myoblast-derived iPSCs for DMD therapy. DMD patient-derived fibroblasts or myoblasts will be reprogramed into iPSCs by reprogramming factors (Oct4, Sox3, Klf4, and cMyc). These fibroblast- and myoblast-derived iPSCs will be induced to myogenic cells via combinatory small molecules and factors such as GSK3βi, forskolin, ascorbic acid, BMPi, ALK5i, Dex, EGF, oncostatin, insulin, bFGF, IGF, HGF, and Wnt3a. These iPSC-derived myogenic cells will be used for autologous cell therapy. Myoblast-derived iPSCs maintain epigenetic myogenic memory.