Crosstalk between Substrates and Rho-Associated Kinase Inhibitors in Cryopreservation of Tissue-Engineered Constructs

Table 2

Effect of substrate on cryopreservation outcome.

Substrate	Reference	Properties
Alginate fibrin beads	Bhakta et al. [7]	(i) Dehydration of beads causes crumpling; (ii) Matricellular environment unaltered; (iii) Cell proliferation and viability not affected; (iv) Highly biocompatible
Chondrite sheets	Maehara et al. [26]	(i) Structural integrity maintained; (ii) Microstructure is not affected; (iii) Membranous structure important for functionality
Alginate-poly-L-lysine	Murua et al. [30]	(i) High chemical resistance and stability; (ii) Efficient therapeutic effect in vivo; (iii) Aided in neovascularization leading to higher haemocrit levels; (iv) Biosafety and long-term functionality of microcapsules
Agarose microencapsulation	Carlos et al. [61]	(i) Young’s modulus reduced; (ii) Diffusion coefficient varied; (iii) Occurrence of cryopreservative damage; (iv) Insulin secretion increment with respect to glucose concentration
Hydroxyapatite scaffolds	Altankov et al. [3]	(i) Detrimental cell surface interaction; (ii) Cell detachment; (iii) Altered thermal properties; (iv) Postthaw viability enhanced correspondingly with number density