Human Umbilical Cord Wharton Jelly-Derived Adult Mesenchymal Stem Cells, in Biohybrid Scaffolds, for Experimental Skin Regeneration

Figure 4

Full-thickness lesion DE grafting. (a) The technique used to implant DE or the scaffold (S) in a full-thickness lesion (W) at the level of the back of the mouse’s neck. The original wound size (1.5 ± 0.1 cm) is indicated by the circle outlined in yellow. S and DE have a suitable resistance to easy suture it to the mouse skin; the same suture thread was used to hold in place the tulle grease medication. (b) Appearance of the lesion treated with the DE in comparison with the simple scaffold (S) or of the not treated wound (W) at three observation times, indicated in yellow, is the original diameter of the wound. The initial delay in the closure of the wound treated with DE is clear. For the observation points of 15 days and 36 days, the appearance of the lesion area on the contrary is also shown. The wound left untreated shows a very thin skin in comparison with the one treated with the DE to 36 days. Tree mice were used for each time point.

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