From Blood to Lesioned Brain: An In Vitro Study on Migration Mechanisms of Human Nasal Olfactory Stem Cells

Figure 3

Inflammation in the lesioned hippocampus. (a) MRI assessment of an in vivo lesion, 24 hours after injection of ibotenic acid in the right hippocampus. Example of an axial contiguous T2-weighted image (slice thickness = 500 μm, TEeff = 60 ms, TR = 3000 ms, rare factor = 8; 8 averages). The hypersignal (bright intensity) in the right hippocampus reveals the extent of the injury. Hoechst blue staining on representative coronal brain sections at the dorsal hippocampal level shows the extent of ibotenic acid-induced neuronal death, one month after the lesion (c), in CA1 and dentate gyrus, in comparison with the unlesioned (b) controlateral hippocampus (white arrows). (d–g) Brain sections were immunostained with anti-Iba1 (in green) and anti-Gfap (in red) antibodies. The four-week-lesioned hippocampus displays numerous activated astrocytes and microglia, in comparison with the healthy controlateral hippocampus. Similar images were obtained with at least three brains. Scale bar: 0.5 mm.

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