From Blood to Lesioned Brain: An In Vitro Study on Migration Mechanisms of Human Nasal Olfactory Stem Cells

Figure 4

Confirmation of cytokine overexpression in the four-week-lesioned hippocampus by RT-qPCR. Scatterplots showing relative expression of selected cytokine transcripts in four-week-lesioned hippocampi (), in comparison with healthy hippocampi (). (a–d) Overexpression of transcript coding for chemotactic cytokines selected after the microarray experiment (i.e., C3, Ccl2, Spp1, and Cxcl10) was confirmed by RT-qPCR. (e–h) Expression of genes known to be overexpressed in the inflamed brain (i.e., Gfap, F4/80, Tnfa, and Il1b) was also assessed. Relative expression levels were determined according to the ΔΔCt method, the Gapdh gene serving as endogenous control for normalization. Each point corresponds to one mouse. The horizontal bars indicate the mean relative gene expression. ( and , using two-tailed Mann–Whitney U test.)

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