Maintenance of a Schwann-Like Phenotype in Differentiated Adipose-Derived Stem Cells Requires the Synergistic Action of Multiple Growth Factors

Figure 4

Forskolin may be involved in the production of BDNF. (a) PCR analysis of BDNF expression shows that both dASC^{+/−forskolin} and dASC^+/−PDGF downregulate their expression of BDNF compared to all other groups (fold change 0.705 ± 0.051 in dASC⁻, 0.302 ± 0.047 in dASC^{+/−forskolin}, 0.761 ± 0.035 in dASC^+/−GGF, 0.749 ± 0.037 in dASC^+/−FGF, and 0.41 ± 0.017 in dASC^+/−PDGF compared to 1 ± 0.044 in dASC⁺. (b) ELISA protein analysis shows that dASC^{+/−forskolin} have a large downregulation of BDNF production compared to all other groups (4.354 ± 0.120 ng/mL in dASC^{+/−forskolin} versus 7.784 ± 0.413 ng/mL in dASC⁻, 12 ± 0.435 ng/mL in dASC^+/−GGF, 10.33 ± 0.197 ng/mL in dASC^+/−FGF, 12.46 ± 0.158 ng/mL in dASC^+/−PDGF, and 14.48 ± 0.128 ng/mL in dASC⁺; , ).

(a)

(b)