Preferential Lineage-Specific Differentiation of Osteoblast-Derived Induced Pluripotent Stem Cells into Osteoprogenitors

<div>Differentiation of hOB-iPSCs into trilineages. (a) hOB-iPSC embryoid bodies (EBs) labeled with antibodies for cytoplasmic germ-layer markers, <i>β</i>-III tubulin (<i>β</i>-III; ectoderm), AFP (endoderm), and SMA (mesoderm). (b) hOB-iPSC EBs labeled with antibodies for germ-layer transcription factors, HAND1 (mesoderm), Sox1 (ectoderm), and Sox17 (endoderm). (c) Undetectable staining for pluripotent cells with TRA-1-60. Scale bars = 40 <i>μ</i>m.</div>

Stem Cells International

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Figure 3

Figure 3: Preferential Lineage-Specific Differentiation of Osteoblast-Derived Induced Pluripotent Stem Cells into Osteoprogenitors 