Preferential Lineage-Specific Differentiation of Osteoblast-Derived Induced Pluripotent Stem Cells into Osteoprogenitors

Figure 5

iOP differentiation to adipocytes, chondrocytes, and osteoblasts. (a) Gene expression of adipocyte, chondrocyte, and osteoblast markers normalized to their respective growth media control cells expression levels. Adipogenic expression levels were lower than both hFB-iOP and hMSC indicating a lack of adipogenic capacity Significantly different from hOB-iOP differentiated cells (). (b) Alizarin Red S, Oil Red O, and Alcian blue staining of adipogenic, osteogenic, and chondrogenic differentiations with a hematoxylin counterstain demonstrated comparable chondrogenic and osteogenic differentiation capacity, with a lack of hOB-iOP adipogenesis. Scale bars = 30 μm.

(a)

(b)