Preferential Lineage-Specific Differentiation of Osteoblast-Derived Induced Pluripotent Stem Cells into Osteoprogenitors

Figure 6

Promoter methylation analysis for unreprogrammed, parental iPSC, and iOP cells. Methylation sensitive enzymatic digestions were performed for hFB and hOB (a), hFB-iPSC and hOB-iPSC (b), and hFB-iOP and hOB-iOP (c). Graphs indicate promoter percent methylated (black) versus percent unmethylated (gray) of a subset of genes: endoderm (FOXA2, STAT1), ectoderm (OLIG2), and mesoderm (NOTCH2, GATA2, PPARγ, RUNX1, and RUNX2).

(a)

(b)

(c)