Stemness Characteristics of Periodontal Ligament Stem Cells from Donors and Multiple Sclerosis Patients: A Comparative Study

Figure 4

Adipogenic differentiation potential of hPDLSCs collected from healthy donors and from MS patients. (a) Photomicrographs of hPDLSCs at P2 (A1) and P15 (B1) under standard conditions and hPDLSCs at P2 (A2) and P15 (B2) at the end of adipogenic commitment. (c) MS-hPDLSCs at P2 (C1) and P15 (D1) under standard conditions and MS-hPDLSCs at P2 (C2) and P15 (D2) at the end of adipogenic differentiation. Differentiation potential was evaluated by the formation of cytoplasmic lipid droplets and vacuoles (appear as cherry red spheres) detected with Oil Red O staining. Mag: 10x. Bar: 10 μm. Adipogenic differentiation capacity was further confirmed by qRT-PCR for adipogenic markers PPARγ and FABP4 in P2 and P15 of (b) hPDLSCs ((A3) and (B3), resp.) and (d) MS-hPDLSCs ((C3) and (D3), resp.). . Data are expressed as mean ± SEM.