Soft Tissue Repair with Easy-Accessible Autologous Newborn Placenta or Umbilical Cord Blood in Severe Malformations: A Primary Evaluation

Figure 2

Proliferation and viability of amnion-residing cells in vitro. Pieces of amnion were kept in culture for up to 21 days in three different culture media and thereafter stained for Ki67 (proliferation) and caspase 3 (apoptosis) at days 0 to 21. There was no significant difference between the culture conditions. A low expression of Ki67 and caspase 3 was detected up to day 14, and on day 21, Ki67 was not detected and caspase 3 showed a slight increased expression. Single positive cells stain brown as indicated by the arrows. Magnification 60x.