Allogeneic Umbilical Cord-Derived Mesenchymal Stem Cells as a Potential Source for Cartilage and Bone Regeneration: An In Vitro Study

Figure 4

Chondrogenic differentiation of UC-MSCs seeded on Hyaff. (a) A representative image of immunofluorescence staining for sox-9 in Hyaff scaffold at low oxygen tension (A) and normoxic (B) conditions. Quantification of the signal is shown in (C): Fluorescence intensity is significantly higher in constructs grown at low oxygen tension (median value and standard deviation: 18 ± 5194; 13 ± 1917 for low oxygen tension and normoxia, resp.). value < 0.05. Magnification 20x; n of samples = 3. (b) A representative image of immunofluorescence staining for collagen type II in Hyaff scaffold at low oxygen tension (A) and normoxic (B) conditions. Signal quantification is shown in (C): Fluorescence intensity is significantly higher in constructs grown at low oxygen tension (median value and standard deviation: 9 ± 2601; 6 ± 1594 for low oxygen tension and normoxia, resp.). value < 0.05. Magnification 20x; n of samples = 3.

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