Allogeneic Umbilical Cord-Derived Mesenchymal Stem Cells as a Potential Source for Cartilage and Bone Regeneration: An In Vitro Study

Figure 7

Expression of osteogenic markers in UC-MSCs seeded onto Orthoss cubes. (a) A representative image of immunofluorescence staining for osteocalcin in Orthoss at 10 (A), 20 (B), and 30 (C) days of in vitro culture. Quantification of the signal is shown in (D): Fluorescence intensity is significantly higher in samples cultured for 30 days (median value and standard deviation: 9604 ± 0.2584; 10.85 ± 0.3990; 13 ± 1030 in samples cultured for 10, 20, or 30 days, resp.). value < 0.05. Magnification 20x; n of samples = 5. (b) A representative image of immunofluorescence staining for RunX-2 in Orthoss at 10 (A), 20 (B), and 30 (C) days of in vitro culture. Quantification of the signal is shown in (D): Fluorescence intensity significantly increases in the longer experimental time points (median value and standard deviation: 7 ± 1340; 11 ± 1712; 18 ± 3090 in samples cultured for 10, 20, or 30 days, resp.). value < 0.05. Magnification 20x; n of samples = 5.

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