Intravenous Administration of Adipose-Derived Stem Cell Protein Extracts Improves Neurological Deficits in a Rat Model of Stroke

Figure 1

ADSCs characterization and ADSC treatment regime. ADSC were isolated from adipose tissue surrounding the enterocoelia. As ADSC approached confluence, they showed a more spindle-shaped, fibroblastic morphology. Scale bars: 100 um (a). (b) ADSC were cultured in the adipogenic induction medium for 14 days. Oil red was used to validate the induction. The intracellular lipid droplets were showed as positive red loci. Scale bars: 100 um (c). ADSC were cultured in the osteogenic induction medium for 21 days. Von Kossa staining showed that a calcified extracellular matrix of induced ADSC was detected. Scale bars: 500 um (d). Flow cytometry analysis was used to measure the surface marker of ADSCs. ADSCs were negative for leukocyte markers, CD45 (1.71%) and CD31 (0.21%), and positive for mesenchymal stem cell markers, CD44 (98.4%) and CD90 (99.9%). (d). ADSC/ADSC-E were administrated through IV or IC or IP 1 hour after tMCAO (e).

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