Insulin-Like Growth Factor Binding Protein-6 Alters Skeletal Muscle Differentiation of Human Mesenchymal Stem Cells

Figure 7

PMSCs under muscle differentiation conditions treated with IGFBP-6 showed more muscle compaction with lower cell count and similar immunofluorescence for MHC compared to cells under muscle differentiation alone at 14 days. Compared to PMSCs cultured under muscle differentiation alone, PMSCs treated with IGFBP-6 showed high cell compaction at day 14 (10x) (a, b) and (c, d) 20x. (e, f) Using immunocytochemistry, PMSCs were immunoreactive for MHC (red-Alexa, λ-568 nm), with no change in immunoreactivity with IGFBP-6 treatment. Nuclei were stained with Hoechst dye (blue, ). Images are representative of 3 technical replicates. (g) PMSCs treated with IGFBP-6 had lower cell count at 14 days postdifferentiation compared to control PMSCs. Data is presented as the mean ± SEM of 15 different fields from 3 independent experiments. One-way ANOVA followed by Student’s t-test, .