Crosstalk with Inflammatory Macrophages Shapes the Regulatory Properties of Multipotent Adult Progenitor Cells

Figure 3

Macrophage-primed rMAPC increase their IL-6 expression. (a) Direct contact cocultures () of macrophages with rMAPC in increasing ratios (1 : 0.5 to 1 : 4) and (b) transwell cocultures of macrophages with rMAPC (1 : 4, experiments), supplemented with LPS. The results are shown as percentage of IL-6 levels normalized to the positive control (1 : 0 +LPS) with duplicates per experiment. Asterisks () indicate statistical significant difference with positive control. (c) Effect of PGE2 (0 to 5 μg/mL) on TNF-α release by macrophages after LPS stimulation ( experiments with triplicates per experiment). The results are shown as percentage of IL-6 levels normalized to the positive control (0 μg/mL +LPS). Asterisks () indicate statistical significant difference with positive control. Mean values of dimethyl sulfoxide are shown as solvent of PGE2 (dotted line). (d) Schematic illustration of generation of rMAPC-conditioned media (SN; supernatant, MΦ; macrophages). (e) IL-6 measurement in rMAPC-derived conditioned media () following different stimuli. IL-6 levels are shown as pg/mL. Asterisks () indicate statistical significant difference between the different conditioned media. (f) IL-6 mRNA expression in rMAPC treated with SN of LPS-activated macrophages, SN of naïve macrophages or LPS ( experiments). The results are shown as fold differences in comparison to SN of naïve macrophages. Asterisks () indicate statistical significant differences. Mean values ± SEM. Data were analyzed with one-way ANOVA followed by Dunnett’s multiple comparison test (, ).

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