Crosstalk with Inflammatory Macrophages Shapes the Regulatory Properties of Multipotent Adult Progenitor Cells

Figure 6

rMAPC suppress antigen presenting features of macrophages. (a) Coculture of macrophages and T cells following macrophages’ pulsing with MBP in specified conditioned media from rMAPC or in their own medium ( experiments with quadruplicates per experiment). T cell proliferation was estimated based to the mean FL1 signal of the proliferated fraction of carboxyfluorescein diacetate succinimidyl ester (CFSE) labeling. The results are shown as percentages of negative control (macrophages cocultured with T cells without prior pulsing of MBP, dotted line). Paragraph signs (§) indicate statistical significant difference between positive control (pulsed macrophages with MBP in fresh medium cocultured with T cells) and negative control. Asterisks () indicate statistical significant difference with positive control. (b) Endocytosis of FITC-labelled beads of macrophages when incubated with designated media. The results are shown as percentages of positive control (endocytosis in fresh medium). Background levels of endocytosis are shown in dotted line. (c) Mean of CD86 expression of macrophages incubated with designated media ( experiments with duplicates per experiment). The results are presented as percentages of positive control (macrophages in fresh medium) (dotted line). Asterisks () indicate statistical significant differences with positive control. (d) Mean of CD86 expression of CD11b/c/CD86 fraction of coculture of macrophages and rMAPC (1 : 4) treated with LPS ( experiments with quadruplicates per experiment). The results are presented as percentages of positive control (1 : 0 +LPS). Asterisks () indicate statistical significant difference with positive control. Mean values ± SEM. Data were analyzed with one-way ANOVA followed by Dunnett’s multiple comparison test or with Kruskal-Wallis test followed by Dunn’s multiple comparison test for nonparametric data. ^§§§, , and .

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