Molecular Genetic Analysis of Human Endometrial Mesenchymal Stem Cells That Survived Sublethal Heat Shock

Figure 1

Karyotype analysis of eMSC. (a) G-banded karyotype of intact eMSC. (b) G-banded karyotype of SHS-survived eMSC. The picture illustrates trisomy and near-centromere breakage of chromosomes 1, 3, monosomy of chromosomes 11; absence of 2 homologs of chromosome 12 is seen in cells after SHS. (c) Molecular karyotyping of intact eMSC; (d) Molecular karyotyping of SHS-survived eMSC; abscissa: length of chromosome 7 in genome, rel. units; ordinate: logarithm of signal intensity, rel. units; arrow shows duplication in chromosomes 7, locus 7q36.3, and size 62 kB; dots on black solid line: deviations in each single base-pair difference in the DNA sequence according to the signal intensity; green solid line: deviations in single base-pair difference in the DNA sequence from the norm with signal intensity combined with genotype.

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