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Stem Cells International
Volume 2017 (2017), Article ID 2374161, 13 pages
Review Article

A Concise Review on the Use of Mesenchymal Stem Cells in Cell Sheet-Based Tissue Engineering with Special Emphasis on Bone Tissue Regeneration

1Department of Orthopaedics and Traumatology, School of Medicine, Pamukkale University, Denizli, Turkey
2Department of Forensic Medicine, Forensic Genetics Laboratory, and Department of Pediatric Genetics, School of Medicine, Ankara University, Ankara, Turkey
3Department of Histology and Embryology, School of Medicine, Ankara Yıldırım Beyazıt University, Ankara, Turkey

Correspondence should be addressed to A. Cevik Tufan

Received 29 June 2017; Revised 30 August 2017; Accepted 12 September 2017; Published 5 November 2017

Academic Editor: Ming Li

Copyright © 2017 A. Cagdas Yorukoglu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The integration of stem cell technology and cell sheet engineering improved the potential use of cell sheet products in regenerative medicine. This review will discuss the use of mesenchymal stem cells (MSCs) in cell sheet-based tissue engineering. Besides their adhesiveness to plastic surfaces and their extensive differentiation potential in vitro, MSCs are easily accessible, expandable in vitro with acceptable genomic stability, and few ethical issues. With all these advantages, they are extremely well suited for cell sheet-based tissue engineering. This review will focus on the use of MSC sheets in osteogenic tissue engineering. Potential application techniques with or without scaffolds and/or grafts will be discussed. Finally, the importance of osteogenic induction of these MSC sheets in orthopaedic applications will be demonstrated.