Allogeneic Adipose-Derived Mesenchymal Stromal Cells Ameliorate Experimental Autoimmune Encephalomyelitis by Regulating Self-Reactive T Cell Responses and Dendritic Cell Function

Figure 3

Allogeneic mASCs reduce demyelinization and inflammatory infiltration in the central nervous system during EAE progression. Mice with MOG-induced EAE were intraperitoneally injected with PBS (control, ) or with mASCs expanded in hypoxia (10⁶ cells/mouse, ) after the onset of the clinical symptoms, and spinal cords were obtained at the peak of the disease 7 days later. ((a) and (b)) Transverse sections of cervical and lumbar regions of spinal cord were randomly analyzed for the presence of inflammatory infiltrates and plaques of demyelinization using the Klüver-Barrera staining (left panels) and anti-myelin basic protein immunohistochemistry (right panels). Arrows point to areas of demyelinization. (c) Gene expression of different inflammatory markers was determined by PCR in mRNAs isolated from the spinal cords. Results are shown as mean (SD) of 4 mice per group. versus control.

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