Research Article
Allogeneic Adipose-Derived Mesenchymal Stromal Cells Ameliorate Experimental Autoimmune Encephalomyelitis by Regulating Self-Reactive T Cell Responses and Dendritic Cell Function
Figure 4
Allogeneic mASCs inhibit MOG-specific immune responses but do not induce foxp3+ Tregs. mASCs were injected intraperitoneally in MOG-induced EAE mice after the onset of the clinical symptoms and spleens and DLNs were isolated at the peak of the disease. Untreated EAE mice were used as controls. (a) The percentages of cytokine-producing cells in spleen and DLNs were determined by intracellular flow cytometry as described in Materials and Methods. ((b) and (c)) DLN and spleen cells were cultured with medium (unstimulated) or stimulated with MOG35-55 (50 μg/mL) or anti-CD3 (1 μg/mL, used as unspecific stimulation). Proliferation was measured after 3 days by [3H]-thymidine incorporation (b). The content of cytokines was determined in culture supernatants after 48 hours (c). (d) The percentages of CD4+Foxp3+ T cells in spleen and DLNs were determined by flow cytometry. Results are shown as mean (SD) from 4 mice per group. versus control.
| (a) |
| (b) |
| (c) |
| (d) |