In Vitro and In Vivo Dentinogenic Efficacy of Human Dental Pulp-Derived Cells Induced by Demineralized Dentin Matrix and HA-TCP

Figure 2

Osteoinduction potentials of HA-TCP and DDM on hDPSCs in vitro. (a) Morphology of hDPSCs cultured in the extracts of HA-TCP and DDM for 3 and 10 days. 1 and 4, hDPSCs cultured in α-MEM with 10% FBS, indicated as Con; 2 and 5, hPDSCs cultured in HA-TCP extract; 3 and 6, hDPSCs cultured in DDM extract. Scale bars: 100 μm. (b) Gene expression of osteogenic and dentinogenic markers in hDPSCs cultured in the extracts of HA-TCP and DDM. Gene expressions of ALP (A), BSP (B), OPN (C), DMP-1 (D), and DSPP (E) were analyzed by the qRT-PCR. 1, actively growing hDPSCs; 2, hDPSCs in the prolonged culture for 10 days; 3, hPDSCs cultured in HA-TCP extract for 10 days; 4, hDPSCs cultured in DDM extract for 10 days. Statistical analyses were carried out using Student’s t-test. ; ; .

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