In Vitro and In Vivo Dentinogenic Efficacy of Human Dental Pulp-Derived Cells Induced by Demineralized Dentin Matrix and HA-TCP

Figure 3

Cell viability of hDPSCs cultured in fibrin gel block. Cells were encapsulated with HA-TCP and DDM in fibrin and cultured in media for 4 days. Fibrin gen blocks were built at a certain size (upper panels). Scale bars: 1 cm. Cell viability was analyzed by using the live-dead viability assay kit. Live and dead cells were stained as green and red, respectively. Cells were detected on the surface of the block (middle panels) and in the interior of the blocks as a cross-sectional view of the block (lower panels). (a) Fibrin gel with hDPSCs. (b) HA-TCP/fibrin gel with hDPSCs. (c) DDM/fibrin gel with hDPSCs. Scale bar: 200 μm.

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