In Vitro and In Vivo Dentinogenic Efficacy of Human Dental Pulp-Derived Cells Induced by Demineralized Dentin Matrix and HA-TCP

Figure 5

Analyses of gene expression of osteoblast markers in transplants. Fibrin blocks of HA-TCP/hDPSCs or DDM/hDPSCs were transplanted in athymic nude mice, and total RNA was isolated from transplant tissues after 8 weeks. Gene expressions of BSP (a), OPN (b), ONT (c), and OCN (d) were detected by qRT-PCR. 1, transplants with HA-TCP with or without hDPSCs; 2, transplants with DDM with or without hDPSCs. Transplantations with and without hDPSCs indicated as –cell and +cell, respectively. Statistical analyses were carried out using Student’s t-test. ; ; ; ; #, significant difference not be detected.

(a)

(b)

(c)

(d)