In Vitro and In Vivo Dentinogenic Efficacy of Human Dental Pulp-Derived Cells Induced by Demineralized Dentin Matrix and HA-TCP

Table 1

Primers used for the quantitative real-time-PCR (qPCR).

Target gene	Primer sequences
Alkaline phosphatase (ALP)	For-5′-CTTGACCTCCTCGGAAGACACTC-3′; Rev-5′-CGCCCACCACCTTGTAGCC-3′
Bone sialophosphoprotein (BSP)	For-5′-TACCGAGCCTATGAAGATGA-3′; Rev-5′-CTTCCTGAGTTGAACTTCGA-3′
Osteopontin (OPN)	For-5′-GTGGGAAGGACAGTTATGAA-3′; Rev-5′-CTGACTTTGGAAAGTTCCTG-3′
Osteonectin (ONT)	For-5′-CTGTTGCCTGTCTCTAAACC-3′; Rev-5′-CACCATCATCAA ATTCTCCT-3′
Osteocalcin (OCN)	For-5′-TGAGTCCTGAGCAGCAG-3′; Rev-5′-TCTCTTCACTACCTCGCT-3′
Dentin matrix protein 1 (DMP1)	For-5′-GACTCTCAAGAAGACAGCAA-3′; Rev-5′-GACTCACTCACCACCTCT-3′
Dentin sialophosphoprotein (DSPP)	For-5′-CAGTACAGGATGAGTTAAATGCCAGTG-3′; Rev-5′-CCATTCCCTTCTCCCTTGTGACC-3’
GAPDH	For-5′-GTATGACAACAGCCTCAAGAT-3′; Rev-5′-CCTTCCACGATACCAAAGTT-3′