Pulsed Electromagnetic Field Regulates MicroRNA 21 Expression to Activate TGF-β Signaling in Human Bone Marrow Stromal Cells to Enhance Osteoblast Differentiation

Table 1

Primers used in this study.

Gene name	Forward primer (5′ > 3′)	Reverse primer (5′ > 3′)
ALP	TGGACGGCCTGGACCTCGTT	AGGGTCAGGAGTTCCGTGCG
COL1A1	GGAGGCACGCGGAGTGTGAG	CCTCTTGGCCGTGCGTCAGG
Osteocalcin	GAGCCCCAGTCCCCTACCCG	GACACCCTAGACCGGGCCGT
FOSB	GCGCCGGGAACGAAATAAAC	TTCGTAGGGGATCTTGCAGC
LEPR	GTGGGGCTATTGGACTGACT	CTTTGAGAGTCCAGCAGGCA
TBRG1	GCTAGATTCCTAGAGGCCCG	GGCATCGGATCCTAAGTCGG
FBN2	CTTTAGGCCGGTTATGCAACG	AATAAGCCCTTCGTCGGCTC
SOX11	TTGGAAGCGGAGAGCAACCT	TGCGTTCGATCTTGGACCAT
CTNNA1	GGCAGCCAAAAGACAACAGG	GGCCTTATAGGCTGCGACAT
AKT3	CTCTATTATTTGGGCTGAGTCATCA	CCCCTCTTCTGAACCCAACC
CXCL12	GACAAGTGTGCATTGACCCG	TGTAAGGGTTCCTCAGGCGT
THBS1	CCTCTACTCCGGACGCAC	GCCCCGGTGAGTTCAAAGAT
COL5A1	CGGGGACTATGACTACGTGC	CTCCAAGTCATCCGCACCTT
GPC4	CAGAGGTCCAGGTTGACACC	TCGGCTTTCTCATTGGCACT
MMP16	TGCGGAACGGAGCAGTATTT	TGTGCTTGTGCTGCCATTTC
TGFB2	CCCCGGAGGTGATTTCCATC	AACTGGGCAGACAGTTTCGG
CDH11	CCCAGTACACGTTGATGGCT	ACGTTCCCACATTGGACCTC
SPP1	GCCTCCTAGGCATCACCTG	CTTACTTGGAAGGGTCTGTGGG
IL8	GGTGCAGTTTTGCCAAGGAG	TTCCTTGGGGTCCAGACAGA
RPL13A	AAGTACCAGGCAGTGACAG	CCTGTTTCCGTAGCCTCATG
hsa-miR-21-5p	UAGCUUAUCAGACUGAUGUUGA