Human Adipose-Derived Stem Cells Exhibit Enhanced Proliferative Capacity and Retain Multipotency Longer than Donor-Matched Bone Marrow Mesenchymal Stem Cells during Expansion In Vitro

Figure 5

Assessment of the influence of monolayer expansion of the osteogenic potential of donor-matched MSCs and ASCs. (a) qPCR analysis of osteogenic marker at ELSC, MLSC, and LLSC in MSCs and ASCs following culture for 21 days in osteogenic media. Data was normalised to the mean of the reference genes MRPL19 and EIF2B1 and presented as relative fold change in gene expression compared to day 0 controls plotted on a log scale. . Values are mean ± SEM. . (b) Quantification of alkaline phosphatase activity in MSCs and ASCs from triplicate wells at ELSC and LLSC. Data represent mean fold change in activity ± SEM compared to day 0 controls. . . (c) Representative images of alizarin red staining of ELSC MSCs and ASCs following 0 days and 21 days in culture in osteogenic media and quantification of alizarin red staining in MSCs and ASCs at ELSC and LLSC. Data represent mean alizarin red concentration ± SEM. . .

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