Human Adipose-Derived Stem Cells Exhibit Enhanced Proliferative Capacity and Retain Multipotency Longer than Donor-Matched Bone Marrow Mesenchymal Stem Cells during Expansion In Vitro

Figure 6

Assessment of the influence of monolayer expansion of the adipogenic potential of donor-matched MSCs and ASCs. (a) qPCR analysis of adipogenic marker genes at ELSC, MLSC, and LLSC in MSCs and ASCs following culture for 21 days in adipogenic media. Data was normalised to the mean of the reference genes MRPL19 and EIF2B1 and presented as relative fold change in gene expression compared to day 0 controls plotted on a log scale. . Values are mean ± SEM. . (b) Representative images of oil red O staining in ELSC and LLSC MSCs and ASCs following 21 days in culture in adipogenic media. (c) Quantification of oil red O staining in MSCs and ASCs from triplicate wells at ELSC, MLSC, and LLSC. Data represents mean oil red O concentration (μg/ml) ± SEM. .

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