Human Adipose-Derived Stem Cells Exhibit Enhanced Proliferative Capacity and Retain Multipotency Longer than Donor-Matched Bone Marrow Mesenchymal Stem Cells during Expansion In Vitro

Table 1

Primer and probe details: gene name, accession number, sequences of forward and reverse primers and probes, and primer concentrations.

Gene name	Accession number	Forward sequence	Reverse sequence	Probe sequence	Primer concentration (nM)
MRPL19	NM_014763	CCACATTCCAGAGTTCTA	CCGAGGATTATAAAGTTCAAA	CAAATCTCGACACCTTGTCCTTCG	900
EIF2B1	NM_001414	TCCCAGATAAGTTTAAGTATAAG	AGCAGAGTGATTAAGGAA	CGCAGACTGGACAAGACCTCA	600
p16^ink4a	NM_000077	CCAGAGGCAGTAACCATG	GACCTTCGGTGACTGATG	AGGTCCCTCAGACATCCCCG	900
p21	NM_000389	CCAGCATGACAGATTTCTA	GAGACTAAGGCAGAAGATG	CGGCTGATCTTCTCCAAGAGGAA	450
p53	NM_000546	CTTGGAACTCAAGGATGC	TTTTATGGCGGGAGGTAG	TTTTGGACTTCAGGTGGCTGGA	450
Nanog	NM_02486	CCTGAAGAAAACTATCCATCC	AGAGGAGAGACAGTCTCC	CAAATGTCTTCTGCTGAGATGCCT	450
RUNX2	NM_001024630	CGCTGCAACAAGACC	CGCCATACAGTAACC	TGGCCTTCAAGGTGGTAGCCCTC	900
ALPL	NM_000478	ACGTCTTCACATTTGGTG	GGTAGTTGTTGTGAGCATA	ACTCTATCTTTGGTCTGGCCCC	450
OCN	NM_199173	CCGCACTTTGCATCG	GCCATTGATACAGGTAGC	CCAGGCAGGTGCGAAGCCC	600
PPARγ	NM_005037	TCTCCACCTTATTATTCTGA	CTGTCATAGATAAGCTTCAA	CGGAAGAAACCCTTGCATCCTT	450
Adipsin	NM_001928	GAAGGTGCAGGTTCTC	CAGGGCCCAGTGTG	TCTCCGACAGCTGTAGCAG	450
LPL	NM_000237	AGGTGATCTTCTGTTCTAG	GCCGTTCTTTGTTCTG	CGCCCAGTTTCAGCCTGAC	600
FABP4	NM_001442	CTGGTACATGTGCAGAAA	ATCCCACAGAATGTTGTA	TCA[+G]TC[+C]AG[+G]TC[+A]ACG	900
SOX-9	NM_000346	GCTCTGGAGACTTCTGAA	GGTACTTGTAATCCGGGTG	TCCTCCTTGTGCTGCACGCG	450
COL2A1	NM_033150	CAGTGGTAGGTGATGTTC	GGCTTCCATTTCAGCTATG	CCAACACTGCCAACGTCCAG	450
ACAN	NM_001135	GGCTTCCACCAGTGTGAC	GTGTCTCGGATGCCATACG	TGACCAGACTGTCAGATACCCCATCCA	900