Research Article

The Angiogenic Potential of DPSCs and SCAPs in an In Vivo Model of Dental Pulp Regeneration

Figure 6

Histological characterization of 3D constructs. (a, f) Human tooth. (b, g) Negative control condition (Puramatrix). (c, h) DPSCs. (d, i) SCAPs. (e, j) DPSCs + SCAPs. In all stem cell constructs (c–e), vascularized pulp-like tissue was formed with a notably lower collagen content in comparison to human dental pulp tissue (a). Negative control constructs also contained vascularized fibrous tissue, albeit with a different organizational structure (b, g). Strongly organized, concentric layers of collagen and mineralized tissue were found in constructs containing stem cells (h–j, arrows). Scale bar = 500 μm (b–e); 200 μm (a, f); 100 μm (j); and 50 μm (g–i). Analysis was conducted on scaffolds with at least 10% newly formed tissue. Representative pictures were taken of four constructs/experimental group.