The Angiogenic Potential of DPSCs and SCAPs in an In Vivo Model of Dental Pulp Regeneration
Tissue and blood vessel content of 3D constructs. (a, b) Negative control condition (Puramatrix). (c, d) DPSCs. (e, f) SCAPs. (g, h) DPSCs + SCAPs. All experimental conditions contained blood vessels of variable shapes and sizes. Scale bar = 500 μm (a, c, e, g); 50 μm (b, d, f, h). Representative pictures were taken of four constructs/experimental group. (i) Percentage of newly formed tissue within the 3D constructs. Negative control constructs contained significantly less tissue in comparison to constructs containing SCAPs. (j) Number of blood vessels with the 3D constructs. Negative control constructs contained a significantly higher amount of blood vessels in comparison to constructs containing DPSCs + SCAPs. (k) Number of blood vessels per mm2 of newly formed tissue. The vascularization rate of the negative control constructs was significantly higher in comparison to that of the constructs containing DPSCs + SCAPs. Analysis was conducted on four constructs/experimental group, with each sample containing more than 10% newly formed tissue. value < 0.05.