Simple Meets Single: The Application of CRISPR/Cas9 in Haploid Embryonic Stem Cells

Table 1

Genetic screens using mouse parthenogenetic haESCs.

Mutagenesis method	Screening strategy	Key factors identified	Reference
PiggyBac transposon	Resistance to 2-amino-6-mercaptopurine to screen for genes involved in DNA mismatch repair	Msh2 and Hprt	[37]
Retroviral gene trap	Resistance to ricin to screen for genes mediating ricin toxicity	Gpr107 and genes in the fucosylation pathway	[38]
PiggyBac transposon	Resistance to 6-thioguanine to screen for genes involved in DNA mismatch repair	Msh2, Msh6, and Mlh1	[53]
	Resistance to olaparib to screen for genes mediating olaparib toxicity	Parp1
PiggyBac transposon	Using a Rex1-GFP reporter to screen for genes required for the exit from self-renewal	Zfp706 and Pum1	[45]
Retroviral gene trap	Using an X-linked inducible Xist gene to screen for genes required for X chromosome inactivation	Spen	[54]
Ethyl methanesulfonate	Resistance to 6-thioguanine to screen for genes involved in DNA mismatch repair	Hprt, Dnmt1, and genes for DNA mismatch repair	[46]