Research Article

Immunoprofiling of Adult-Derived Human Liver Stem/Progenitor Cells: Impact of Hepatogenic Differentiation and Inflammation

Figure 2

CD200 expression in naïve ADHLSC and mature nonplated hepatocytes. (a) Total RNA extracted from both naïve ADHLSCs and nonplated hepatocytes was retrotranscribed, the cDNA-synthesized and PPIA-normalized CD200 mRNA levels were evaluated by RT-qPCR. A very significant low level of this marker is noticed in mature hepatocytes () ( versus naïve ADHLSC, unpaired t-test). (b) Cell extracts were prepared and immunoblotted with anti-CD200 and anti-GAPDH antibodies as described in Materials and Methods. Low CD200 protein expression in mature hepatocytes () is correlated to mRNA expression as compared to ADHLSC. Ponceau S staining shows the quality and the quantity of loading. (c) Immunofluorescence on fixed naïve ADHLSCs was also performed to appreciate the CD200 protein expression. CD200-positive immunoreactivity was significantly confirmed. The lower picture shows that no immunofluorescence signal was observed when cells were incubated with only secondary antibody. Pictures from 3 different donors were taken at a magnification of 200x.