Signal Factors Secreted by 2D and Spheroid Mesenchymal Stem Cells and by Cocultures of Mesenchymal Stem Cells Derived Microvesicles and Retinal Photoreceptor Neurons

Figure 4

Characteristics of 2D-MSC-MVs and 3D-MSC-MVs. (a)–(d) By electron microscopy, both 2D-MSC-MVs (a, c) and 3D-MSC-MVs (b, d) displayed sizes ranging from 100 nm to 400 nm. Scale bar = 200 nm, 1 um. (e)–(g) Light microscopy analyses showed that, similar to controls (e), both of 2D-MSC-MVs (f) and 3D-MSC-MVs (g) could maintain the normal shape of cocultured 661W cells (scale bar = 200 um).

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