Identification of Stem Leydig Cells Derived from Pig Testicular Interstitium

Figure 2

Identification of porcine SLCs. (a) The primary SLCs isolated from 7-day-old porcine testes with the help of collagenase type IV (bar = 50 μm). (b) RT-PCR results of genes involved in stem cells potential and spermatogenesis; SLCs, the pig primary isolated SLCs; +, positive control (7-day-old pig testes); −, negative control (sterile water). (c and d) Expressions of LIFR and PDGFRα in pig testes, pig Sertoli cells, pig Spermatogonia stem cells and pig primary isolated SLCs as fold change relative to beta-actin; Spermatogonia, Spermatogonia stem cells. Different letters (A, B) indicate significant difference ().

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