Research Article

Human ADMC-Derived Adipocyte Thermogenic Capacity Is Regulated by IL-4 Receptor

Figure 3

Effect of IL-4 on the expression of thermogenic genes. Precursor cells were obtained, proliferated, and induced to differentiate into mature adipocytes and then treated with BSA alone (vehicle) or IL-4. (a) UCP-1 mRNA expression was measured by qPCR. (b) Protein expression of PGC-1α, UCP-1, and CITED1 was measured by Western blotting. (c) Relative band intensities for each were determined by densitometry. Statistical analyses were performed using ANOVA. Data represent mean ± SD and were normalized to GAPDH as reference gene/protein. between precursors (SVF) and mature adipocytes (MA) after 6 h of IL-4 treatment, relative RNA expression between mature adipocytes treated with vehicle or IL-4, and # between mature adipocytes treated with IL-4 or vehicle.
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