Research Article
Human ADMC-Derived Adipocyte Thermogenic Capacity Is Regulated by IL-4 Receptor
Figure 5
Effect of IL-4 on adipocytokine expression by adipocytes. Precursor cells were obtained, proliferated, and induced to differentiate into mature adipocytes, then treated with BSA alone (vehicle) or IL-4. (a) Adiponectin, FGF21, leptin, and FAB4 protein expression were measured by Western blotting. (b) Relative band intensity of each was determined by densitometry. Statistical analyses were performed using ANOVA. Data represent mean ± SD and were normalized to the reference protein GAPDH. between precursors (SVF) and mature adipocytes (MA). # between mature adipocytes (MA) treated with IL-4 and vehicle.
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