Paracrine Secreted Frizzled-Related Protein 4 Inhibits Melanocytes Differentiation in Hair Follicle

Figure 5

Effects of sFRP4 on Wnt3a-induced differentiation in melanocytes in vitro. (a) JB6 epithelial cells were infected with AdsFRP4, AdWnt3a, and AdGFP, respectively, or coinfected with AdWnt3a and AdsFRP4. Then JB6 cells and iMC23 melanocyte progenitors were cocultured. (b) Direct observation of melanogenesis of iMC23 cells 5 days after being cocultured with JB6 cells. (c) Masson-Fontana staining reveals the melanin granules formation. Bars: 100 um. (d) Tyrosinase activity assay of iMC23 cells 3 days after coculture. The values indicate the mean of three independent experiments ± SD. . (e) Western blot and statistical analysis reveal the protein expression levels of TRP2, TRP1, tyrosinase, and β-catenin in different treatment groups.

(a)

(b)

(c)

(d)

(e)