Figure 2: miR-214 expression decreased after osteogenic induction. (a) Osteogenic induction was verified by increased expression of RUNX2, ALP, and OCN in mRNA level. (b) Alizarin red staining was performed at 21 days. (c) qRT-PCR was used to quantify miR-214 absolute (left) and relative (right) expression levels after 7 days of osteogenic differentiation. Absolute quantitation was normalized to 100 U6 copies. (d) After transfection of inhibitor-214 for 72 h, qRT-PCR was conducted to detect osteogenesis gene markers. and .