Research Article

MicroRNA-214 Suppresses Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Targeting ATF4

Figure 3

miR-214 directly targets ATF4. (a) Diagram of luciferase reporters, wild-type ATF4 3′UTR (WT), or mutant ATF4 3′UTR (Mut). (b) HEK-293T cells were transfected with mimic-214 and qPCR was performed after 36 h. (c) The effect of mimic-214 with ATF4-3′UTR-WT/Mut on luciferase activity in HEK-293T cells. The luciferase/renilla activity of the groups was calculated. .