The Impact of GFP Reporter Gene Transduction and Expression on Metabolomics of Placental Mesenchymal Stem Cells Determined by UHPLC-Q/TOF-MS

Figure 2

The 9/1 methanol (MeOH)/chloroform (CHCl₃) and the 1/1 MeOH/H₂O solvents were used to extract metabolites from the hPMSCs and GFP⁺hPMSCs. The extracts obtained by the two methods were assayed by positive-ion ultra-high performance liquid chromatography-electrospray ionization ultra-mass spectrometry (UHPLC-ESI-MS). A heat map was generated from the liquid chromatography-mass spectrometry (LC-MS) data by the hierarchical clustering algorithm. The hierarchical clusters were calculated from the individual values using 1 Pearson correlation coefficient as distance and complete linkage for agglomeration. Each data point represents the relative intensity of the metabolite in each sample. The red colors presented in the heat map indicate values above the normalized average value, while blue indicates those below the standard value. The color shade is directly proportional to the intensity.