Inhibition of Histone Methyltransferase, Histone Deacetylase, and β-Catenin Synergistically Enhance the Cardiac Potential of Bone Marrow Cells

Figure 10

Model of epigenetic and Wnt regulation of cardiogenesis. Results presented in this study indicate that BIX01294 induces a precardiac phenotype from MSCs and acts cooperatively with Wnt11 in promoting both cardiac differentiation and inhibiting the intracellular accumulation of β-catenin. Inhibition of β-catenin accumulation by cells of the early mesoderm has been postulated to be a key element in the initiation of heart formation. Thus, we hypothesize that the BIX01294 prerequisite for generating cardiac phenotypes from MSCs is twofold: (1) to promote gene expression that is in accordance with a precardiac phenotype and (2) cooperatively allow Wnt11 to reduce cellular β-catenin levels, thereby initiating cardiac differentiation of the treated MSCs.