Osteogenic Differentiation of hDPSCs on Biogenic Bone Apatite Thin Films

Figure 5

(a) Immunofluorescence analysis performed on undifferentiated hDPSCs and on osteogenic differentiated hDPSCs cultured on BAp_HT films for 3 weeks in standard culture conditions and osteogenic medium, respectively. Representative images show the expression and cellular localization of Runx-2, OSX, and OCN. DIC images report film adhesion at 3 weeks of culture and hDPSC distribution on the film surface. Nuclei were counterstained with DAPI. Scale bar: 10 μm. (b) Western blot analysis was performed on undifferentiated and osteogenic differentiated hDPSCs grown on control glass coverslips and on BAp_HT films, respectively, for Runx-2 and OCN expression. Histograms represent densitometry analysis of Runx-2 and OCN. Actin bands were used as loading control. , differentiated hDPSCs versus undifferentiated hDPSCs grown on the two different surfaces; ^# differentiated hDPSCs on control coverslips versus differentiated hDPSCs on BAp_HT film; undifferentiated hDPSCs on BAp_HT films versus undifferentiated hDPSCs on control coverslips; differentiated hDPSCs on BAp_HT films versus differentiated hDPSCs on control coverslips.

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