Human Menstrual Blood-Derived Mesenchymal Stem Cells as Potential Cell Carriers for Oncolytic Adenovirus

Figure 2

MenSC infection and viral replication. (a) Infectivity assay in MenSCs. MenSCs (5 × 10⁴ cells/well) were infected for 24 hours at 37°C with AdGL, AdGLRGD, and AdGLK at different multiplicities of infection (25, 10, 5, and 1 TU/cell). Infected cells were analyzed by flow cytometry. Results are shown as the % of infected cells (green cells). Mean values () ± SD are represented. (b) MenSCs were analyzed by flow cytometry for the expression of the main adenovirus type 5 receptors. MenSCs express αv-integrins but do not express the coxsackievirus B and adenovirus receptor (CAR). (c) OAdv production kinetics and release in A549 cells, BM-MSCs, and MenSCs. Extracellular (SN) and total virus produced (CE) were measured at the indicated time points. Mean values () ± SD are plotted. (d) Cytotoxicity analysis of OAdv produced by MenSCs. A549, Panc-01, NP-18, and SKmel28 cancer cell lines were cocultured with previously infected MenSCs (MOI of 50 TU/cell of ICOVIR15 for 24 h before coculture) at different infected MenSCs : cancer cell ratios. At day 5 of coculture, cell survival was measured and IC50 values calculated. Mean values () ± SD are plotted.

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