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Stem Cells International
Volume 2017, Article ID 3615729, 10 pages
Research Article

Human Menstrual Blood-Derived Mesenchymal Stem Cells as Potential Cell Carriers for Oncolytic Adenovirus

1Virotherapy and Gene Therapy Group, ProCure Program, Translational Research Laboratory, Instituto Catalan de Oncología-IDIBELL, Barcelona, Spain
2Servei d'Anatomia Patològica, Hospital Universitari Arnau de Vilanova i Institut de Recerca Biomèdica de Lleida, Lleida, Spain
3Genomics and Cytomics Unit, Bellvitge Biomedical Research Institute (IDIBELL), Barcelona, Spain
4Cellular Biotechnology Laboratory, Institute of Health Carlos III (ISCIII), Majadahonda, Madrid, Spain

Correspondence should be addressed to R. Moreno; ten.aigolocnoci@oneromafar

Received 16 February 2017; Revised 19 April 2017; Accepted 4 May 2017; Published 11 July 2017

Academic Editor: Gerald A. Colvin

Copyright © 2017 R. Moreno et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure 1. Morphologic and phenotypic characterization of Menstrual Blood-Derived Mesenchymal Stem Cells. A) Phase-contrast microscopy image of mesenchymal stem cells isolated from menstrual blood (MenSCs). Cells show a spindle-shaped fibroblast-like morphology. The MenSCs population was induced with the respective differentiation media for 3–4 weeks. B) The lipid droplets present into adipocytes were stained with Oil Red-O (left, control non-differentiated stained MenSCs, right). C) Chondrocyte mucopolysaccharides were observed with Alcian Blue staining (left, control non-differentiated stained MenSCs, right). The calcification of osteocytes was observed through Alizarin Red staining (left, control non-differentiated stained MenSCs, right) D). Magnification 20x; scale bar: 10μm. Supplementary Figure 2. Representative flow cytometry analysis of MenSCs immunophenotype. While MenSCs strongly express mesenchymal stem cell antigens (CD29, CD90, CD44, CD73 and CD105) they do not express neither hematopioetic markers (CD34, HLA-DR, CD133 and CD14) nor co-stimulatory proteins (CD40, CD83, CD86).

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