A Conditioned Medium of Umbilical Cord Mesenchymal Stem Cells Overexpressing Wnt7a Promotes Wound Repair and Regeneration of Hair Follicles in Mice

Figure 7

-CM enhances migration and expression of the cytoskeletal components K6 and K16 in human keratinocytes. (a) Typical images of dorsal skin showing effectiveness of wound closure in the mice and H&E-stained histological slices of treatments groups -CM, -CM, and HF-CM. (b) Evaluation of wound closure by morphometrical analysis of wound areas (ImageJ, NIH). (c and d) -CM enhanced migration of human keratinocytes in a wound scratch assay after 24 h as compared to -CM, HF-CM, and DMEM and corresponding semiquantitative analysis data. (e) Expression levels of c-Myc, K6, and K16 mRNA after treatment with -CM, -CM, or HF-CM were analyzed by real-time PCR and normalized to that of GAPDH. (f, g) Western blots analysis of c-Myc, K6, K16, and corresponding semiquantitative analysis. The data represent mean ± SEM, ; and . Solid lines indicate the initial wound area; dotted lines demarcate the migrating front of the cells. The scale bar is 100 μm.

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