A Conditioned Medium of Umbilical Cord Mesenchymal Stem Cells Overexpressing Wnt7a Promotes Wound Repair and Regeneration of Hair Follicles in Mice
Table 1
List of sequences of forward and reverse primers.
Genes
Species
Forward primer
Reverse primer
α-SMA
Human
5′-CTATGAGGGCTATGCCTTGCC-3′
5′-GCTCAGCAGTAGTAACGAAGGA-3′
Collagen I
Human
5′-GTGCGATGACGTGATCTGTGA-3′
5′-CGGTGGTTTCTTGGTCGG-3
Collagen III
Human
5′-TTGAAGGAGGATGTTCCCATCT-3′
5′-ACAGACACATATTTGGCATGGT-3′
K6
Human
5′-ACGGAAACTACTACGGCGAC-3′
5′-GGCCTTCGTATCCACAGCAC-3′
K16
Human
5′-GACCGGCGGAGATGTGAAC-3′
5′-CTGCTCGTACTGGTCACGC-3′
C-Myc
Human
5′-AATAGAGCTGCTTCGCCTAGA-3′
5′-GAGGTGGTTCATACTGAGCAAG-3′
GAPDH
Human
5′-CTGGGCTACACTGAGCACC-3′
5′-AAGTGGTCGTTGAGGGCAATG-3′
α-SMA
Mouse
5′-GTCCCAGACATCAGGGAGTAA-3′
5′-TCGGATACTTCAGCGTCAGGA-3′
Collagen I
Mouse
5′-GCTCCTCTTAGGGGCCACT-3′
5′-CCACGTCTCACCATTGGGG-3′
Collagen III
Mouse
5′-CTGTAACATGGAAACTGGGGAAA-3′
5′-CCATAGCTGAACTGAAAACCACC-3′
GAPDH
Mouse
5′-AGGTCGGTGTGAACGGATTTG-3′
5′-TGTAGACCATGTAGTTGAGGTCA-3′
PCR amplification conditions on the Applied Biosystems 7500 Real-Time PCR System: 58°C for 5 minutes; 95°C for 2 minutes; 40 cycles of 95°C for 15 seconds and 60°C for 60 seconds.
